Abstract
The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa
were localized using fluorescent in situ hybridization (FISH).
Two 18S-28S rDNA sites and one 5S rDNA site were identified
and located at the ends of 3 different chromosomes.
FISH signals from the Arabidopsis -type telomere repeat sequence
were observed at the distal ends of each chromosome.
Six BAC clones selected from 2 linkage groups based
on genome sequence assembly (LG-I and LG-VI) were localized
on 2 chromosomes, as expected. BACs from LG-I hybridized
to the longest chromosome in the complement. All
BAC positions were found to be concordant with sequence
assembly positions. BAC-FISH will be useful for delineating
each of the Populus trichocarpa chromosomes and improving
the sequence assembly of this model angiosperm tree
species.
