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By engineering the Serine Integrase Assisted Genome Engineering (SAGE) genetic toolkit in an industrial strain of Aspergillus niger, we have established its proof of principle for applicability in Eukaryotes.
This technology can activate gene expression in a time- and dose-dependent manner in the thermophilic bacterium Clostridium thermocellum. This system will mediate inducible gene expression for strain engineering in C.